Strategic Research Centre Nacional d'Anàlisi Genòmica CNAG-CRG

Centre Nacional d'Anàlisi Genòmica CNAG-CRG

The CNAG's mission is to carry out projects in DNA sequence analysis in collaboration with the Spanish, European and International Research Community. Since the 1st July 2015 the CNAG is integrated with the CRG. The center has one of the largest DNA sequencing capacities in Europe (1000 Gbases of sequencing data per day), and an extensive informatics infrastructure.

The CNAG focuses its research efforts on the development of analytical and bioinformatic tools for next generation sequencing and on the analysis and interpretation of genome information in areas such as cancer genetics, rare disorders, host-pathogen interactions, the preservation of endangered species, evolutionary studies, 3D genomics, epigenomics and improvement of species of agricultural interest. Researchers at CNAG participate in major national and international collaborative research projects in genome research such as ICGC, IRDiRC, GA4GH, ELIXIR and IHEC.

Main projects

  • 1.


    RD-Connect is a global infrastructure project funded by EU that links genomic data with registries, biobanks, and clinical bioinformatics tools to produce a central research resource for rare diseases (RD). Within this project, the CNAG has developed a highly sophisticated system to combine data from different ‑omics levels in order to facilitate gene and biomarker discovery through efficient annotation systems and expert systems able to extract knowledge from data.

  • 2.


    The EU funded B-CAST project aims to generate new data on molecular characterisation of ~20,000 tumours from large-scale epidemiological studies, clinical studies and biobanks. The data will be utilised in a statistical model to calculate individualised risk scores to develop certain (sub) types of breast cancer.

  • 3.


    The aim of this ERC-Synergy project is to study the conformation of the genome at various integrated levels, from the nucleosome fiber to the distribution of chromosomes territories in the nuclear space, and to explore whether the genome 3D structure acts as an information source for modulating transcription in response to external stimuli.



Bioinformatics expertise:

Group Leader:

Ivo Glynne Gut


Bioinformatics services offered

  • Primary run analysis and quality control

  • Alignment of sequencing reads to the reference sequence

  • Variant calling and annotation pipelines, including SNVs, InDels and CNVs

  • Proprietary variant filtration and prioritization tools.

  • Proprietary genome assembly pipeline using shotgun genome sequencing and/or fosmid pool approaches.

  • Proprietary genome annotation pipeline for coding and non-coding RNAs.

  • Differential expression analysis pipeline including proprietary GEM-split-mapper and Flux Capacitor for gene and isoform quantification.

  • Identification of gene fusions from transcriptome reads.

    more information at